Studies upon the Sulfhydryl Groups of Calf Lens α-Crystallins
نویسندگان
چکیده
منابع مشابه
Age-dependent association of γ-crystallins with aged α-crystallins from old bovine lens
PURPOSE Previous theoretical and experimental studies have predicted that the loss of weak protein interactions between alpha- and gamma-crystallins could result in a decrease in the transparent properties of the aging lens. METHODS alpha-Crystallins were prepared from the nucleus of old bovine lens, and gamma-crystallins were prepared from whole fetal bovine lens or from the nucleus of old b...
متن کاملStudies on lens proteins. I. Subunit structure of beta crystallins of rabbit lens cortex.
A method has been developed to isolate and characterize beta-crystallins of rabbit lens cortex. Chromatographic separation of water-soluble structure proteins of rabbit lens cortex on a Sephacryl S-200 gel column yielded four beta-crystallin peaks (beta1, beta2, beta3 and beta4), all eluting between alpha and gamma-crystallins. Their molecular weights were estimated to be 250,000, 130,000, 60,0...
متن کاملStudies on lens proteins. III. Variations in polypeptides of lens beta-crystallins.
Relative amounts of two water-soluble beta-crystallin polypeptides with molecular weights of 26,000 (26K) and 28,000 daltons (28K) were measured in a number of species and also in different age groups of rats. The data indicate an age-dependent increase in the quantity of the 26K which is concomitant with a decrease in the 28K polypeptide. The ratio of these two polypeptides in the total water-...
متن کاملα- and β-crystallins modulate the head group order of human lens membranes during aging.
PURPOSE To examine the physical properties of human lens cell membranes as a function of age. METHODS The environment of the phospholipid head groups in fiber cell membranes from human lenses, aged 22 to 83 years, was assessed with Laurdan and two-photon confocal microscopy. The effect of mild thermal stress on head group order was studied with lens pairs in which one intact lens was incubate...
متن کاملAssociation of partially folded lens βB2-crystallins with the α-crystallin molecular chaperone
Age-related cataract is a result of crystallins, the predominant lens proteins, forming light-scattering aggregates. In the low protein turnover environment of the eye lens, the crystallins are susceptible to modifications that can reduce stability, increasing the probability of unfolding and aggregation events occurring. It is hypothesized that the α-crystallin molecular chaperone system recog...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1967
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)95850-3